GO注释突然遇到一个报错：
09/08/2023 13:53:59:226 Welcome to InterProScan-5.36-75.0
09/08/2023 13:54:04:551 Running InterProScan v5 in STANDALONE mode... on Linux
09/08/2023 13:54:16:129 Loading file /GO/split_pep/split.01.fa
09/08/2023 13:54:16:134 Running the following analyses:
[CDD-3.17,Coils-2.2.1,Gene3D-4.2.0,Hamap-2019_01,MobiDBLite-2.0,PANTHER-14.1,Pfam-32.0,Phobius-1.01,PIRSF-3.02,PRINTS-42.0,ProSitePatterns-2019_01,ProSiteProfiles-2019_01,SFLD-4,SignalP_EUK-4.1,SignalP_GRAM_NEGATIVE-4.1,SignalP_GRAM_POSITIVE-4.1,SMART-7.1,SUPERFAMILY-1.75,TIGRFAM-15.0,TMHMM-2.0c]
Pre-calculated match lookup service DISABLED. Please wait for match calculations to complete...
09/08/2023 13:54:49:322 25% completed
09/08/2023 13:58:34:783 51% completed
2023-08-09 14:05:12,501 [amqEmbeddedWorkerJmsContainer-2] [uk.ac.ebi.interpro.scan.management.model.implementations.RunBinaryStep:188] ERROR - Command line failed with exit code: 1
Command: bin/tmhmm/2.0c/decodeanhmm data/tmhmm/2.0c/TMHMM2.0c.model /GO/blast_out/tempdir01/compute-b07-8.hpc_20230809_135404545_a9j4//jobTMHMM/000000000001_000000000212.fasta -N 1 -PrintNumbers -background 0.081 0.015 0.054 0.061 0.040 0.068 0.022 0.057 0.056 0.093 0.025 0.045 0.049 0.039 0.057 0.068 0.058 0.067 0.013 0.032
Error output from binary:
decodeanhmm 1.1g
Copyright (C) 1998 by Anders Krogh
Wed Aug 9 14:05:12 2023
Error: Couldn't open file 0000000001_000000000212.fasta
2023-08-09 14:05:12,505 [amqEmbeddedWorkerJmsContainer-2] [uk.ac.ebi.interpro.scan.jms.worker.LocalJobQueueListener:204] ERROR - Execution thrown when attempting to executeInTransaction the StepExecution. All database activity rolled back.
java.lang.IllegalStateException: Command line failed with exit code: 1
Command: bin/tmhmm/2.0c/decodeanhmm data/tmhmm/2.0c/TMHMM2.0c.model /GO/blast_out/tempdir01/compute-b07-8.hpc_20230809_135404545_a9j4//jobTMHMM/000000000001_000000000212.fasta -N 1 -PrintNumbers -background 0.081 0.015 0.054 0.061 0.040 0.068 0.022 0.057 0.056 0.093 0.025 0.045 0.049 0.039 0.057 0.068 0.058 0.067 0.013 0.032
Error output from binary:
decodeanhmm 1.1g
Copyright (C) 1998 by Anders Krogh
Wed Aug 9 14:05:12 2023
Error: Couldn't open file 0000000001_000000000212.fasta
.............略略略，后边我就不贴了哈！
主要报错信息还是：
ERROR - Command line failed with exit code: 1
如何解决呢？！
报错的原因是：
MHMM 中有一个错误，如果 fasta 文件路径超过 230 个字符，它会抛出如上的错误。
解决方式：
1）使用不是很长的目录路径，以便最终路径仍然少于 230 个字符
或
2）使用Phobius，因为Phobius将为您提供使用TMHMM时获得的几乎所有预测
由于第1个方法更好操作，当然选它！选它！选它！
有尝试过第2个方法的宝子欢迎来补充哈。。。
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